从健康蛋鸡和腹泻患者中分离弯曲杆菌属的不同程序、运输培养基和增菌培养基的比较
Comparison of different procedures, transport media, and enrichment media for isolation of Campylobacter species from healthy laying hens and humans with diarrhea.
作者信息
Sjögren E, Lindblom G B, Kaijser B
机构信息
Department of Clinical Bacteriology, University of Göteborg, Sweden.
出版信息
J Clin Microbiol. 1987 Oct;25(10):1966-8. doi: 10.1128/jcm.25.10.1966-1968.1987.
Abstract
A comparison was made with some of the available transport and enrichment media for the isolation of Campylobacter species. The merits of duplicate versus single sampling were also investigated. Fecal samples from 721 healthy laying hens and 328 patients with diarrhea were included in the study. The media compared were modified Stuart medium, Campythio-glycolate medium, semisolid motility test medium, and Campylobacter enrichment broth. The isolation rate of Campylobacter species was significantly increased by taking duplicate instead of single samples. The highest outcome was found when one of the two samples was an enrichment medium, such as semisolid motility test medium.
摘要
对一些用于分离弯曲杆菌属的现有运送和增菌培养基进行了比较。还研究了重复采样与单次采样的优缺点。本研究纳入了721只健康蛋鸡和328例腹泻患者的粪便样本。所比较的培养基有改良斯图尔特培养基、弯曲硫乙醇酸盐培养基、半固体动力试验培养基和弯曲杆菌增菌肉汤。通过采集重复样本而非单次样本，弯曲杆菌属的分离率显著提高。当两个样本之一为增菌培养基（如半固体动力试验培养基）时，分离效果最佳。
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