Kenneally D, Mackay I R, Toh B H
J Clin Lab Immunol. 1984 May;14(1):13-6.
Antinucleolar antibody (ANoAb) was tested for in sera from 25 patients with systemic lupus erythematosus (SLE), 61 with progressive systemic sclerosis (PSS), 22 with chronic active hepatitis (CAH) and 28 healthy persons, using immunofluorescence reactivity with acetone-fixed monolayers of cultured human fibroblasts, and a procedure to reveal ANoAb when other antinuclear antibodies were concurrently present. ANoAb was found on direct testing in sera from 6 patients with SLE, 15 with PSS and 7 with CAH, but not in any of 28 sera from healthy persons; homogeneously reactive antinuclear antibody was also present in the serum of these 6 cases of SLE, in 6 of the 15 with PSS and in 3 of the 7 with CAH and, in SLE specifically, pre-treatment of fibroblast monolayers with DNase "unmasked" the presence of ANoAb in a further 7 sera which had shown only homogeneous nuclear staining in fibroblasts. ANoAb belonged to the IgG, IgM and IgA class in sera from cases of SLE and PSS, and to only the IgG and IgM class in sera from cases of CAH. ANoAb titres were highest in patients with PSS. ANoAb were sensitive to RNase in 5 cases, to RNase and DNase in 6, and were sensitive to combinations of RNase, DNase, NaC1, and trypsin in the remaining cases. We conclude that (i) fibroblast monolayers are a suitable substrate for the demonstration of ANoAb, (ii) homogeneous staining of cell nuclei may mask ANoAb, so that the incidence of ANoAb becomes higher in SLE than in PSS, (iii) low-titre ANoAb in CAH not visualized in frozen tissue sections may be detected on fibroblast monolayers, and (iv) nucleolar antigens probably include RNA, RNA bound to DNA, and RNA bound to proteins.
采用培养的人成纤维细胞丙酮固定单层免疫荧光反应法，以及在同时存在其他抗核抗体时检测抗核仁抗体（ANoAb）的方法，对25例系统性红斑狼疮（SLE）患者、61例进行性系统性硬化症（PSS）患者、22例慢性活动性肝炎（CAH）患者和28名健康人的血清进行了ANoAb检测。直接检测发现，6例SLE患者、15例PSS患者和7例CAH患者的血清中有ANoAb，但28名健康人的血清中均未检测到；这6例SLE患者、15例PSS患者中的6例以及7例CAH患者中的3例血清中还存在均匀反应性抗核抗体，并且在SLE患者中，用脱氧核糖核酸酶（DNase）预处理成纤维细胞单层后，又有7例血清中“显示”出了ANoAb，这些血清在成纤维细胞中原本仅显示均匀的核染色。SLE和PSS患者血清中的ANoAb属于IgG、IgM和IgA类，而CAH患者血清中的ANoAb仅属于IgG和IgM类。PSS患者的ANoAb滴度最高。5例患者的ANoAb对核糖核酸酶（RNase）敏感，6例对RNase和DNase敏感，其余患者的ANoAb对RNase、DNase、氯化钠（NaCl）和胰蛋白酶的组合敏感。我们得出以下结论：（i）成纤维细胞单层是检测ANoAb的合适底物；（ii）细胞核的均匀染色可能掩盖ANoAb，因此SLE患者中ANoAb的发生率高于PSS患者；（iii）在冷冻组织切片中未显示的CAH患者中的低滴度ANoAb，在成纤维细胞单层上可能被检测到；（iv）核仁抗原可能包括RNA、与DNA结合的RNA以及与蛋白质结合的RNA。
