Mathy J P, Baum R, Toh B H
Clin Exp Immunol. 1980 Jul;41(1):73-80.
Sera from a patient with systemic lupus erythematosus (SLE), tested by indirect immunofluorescence on frozen tissue sections, gave granular cytoplasmic staining of hepatocytes, gastric chief cells, exocrine cells of the pancreas and submandibular glands, and cerebellar Purkinje cells. In acetone-fixed monolayers of rat embryonic fibroblasts, 3T3 cells, mouse neuroblastoma cells, and cells from a human melanoma and colon carcinoma cell line, the sera stained perinuclear cytoplasmic granules which radiated out towards the cell periphery. More mature and differentiated fibroblasts from rat of human foetal lung showed staining of reticular cytoplasmic structures corresponding to phase-dense rough endoplasmic reticulum (RER). Nucleoli were prominently stained in all cultured cells. Serum absorption with ribosomes inhibited all antibody activity but absorption with RNA or with RNase-treated ribosomes resulted only in partial inhibition. Monolayers of RNase-treated fibroblasts gave weaker staining reactions compared to control untreated cultures. These observations suggest that the autoantibody is directed against ribosomal RNA and ribosomal protein present in cytoplasmic polyribosomes, in RER and in nucleoli.
通过对冰冻组织切片进行间接免疫荧光检测，来自一名系统性红斑狼疮（SLE）患者的血清对肝细胞、胃主细胞、胰腺和下颌下腺的外分泌细胞以及小脑浦肯野细胞呈现颗粒状细胞质染色。在大鼠胚胎成纤维细胞、3T3细胞、小鼠神经母细胞瘤细胞以及人黑色素瘤和结肠癌细胞系的丙酮固定单层细胞中，血清对向细胞周边辐射的核周细胞质颗粒进行染色。来自人胎儿肺的大鼠更成熟和分化的成纤维细胞显示出与相致密粗面内质网（RER）相对应的网状细胞质结构的染色。在所有培养细胞中核仁均显著染色。用核糖体吸收血清可抑制所有抗体活性，但用RNA或经核糖核酸酶处理的核糖体吸收血清仅导致部分抑制。与未处理的对照培养物相比，经核糖核酸酶处理的成纤维细胞单层的染色反应较弱。这些观察结果表明自身抗体针对的是存在于细胞质多核糖体、RER和核仁中的核糖体RNA和核糖体蛋白。
