Tsuchiya T, Yamaha T
Mutat Res. 1984 Aug;130(4):267-72. doi: 10.1016/0165-1161(84)90102-x.
Mutagenicity was detected using Salmonella typhimurium TA98 in the acidic components of the intestinal contents of mice, in which a high incidence of hepatoma had been reported due to feeding on a diet containing 15% sorbic acid (Ishizawa et al., 1980). Furthermore, the glutathione level in the liver of the 15% sorbic acid group was decreased to 40% of the amount found in controls after a 3-month feeding period, and this low level was maintained for long periods (12 months) until the end of the experiments. There was also a close correlation between the extent of depletion of the hepato-glutathione level and the concentration of sorbic acid added to the diet. Consequently, the hepatoma which developed in mice fed a 15% sorbic acid diet was considered to be induced both by the depletion of the hepato-glutathione level over the long periods, and by the gradual production of various mutagens in the intestine which were absorbed and transferred to liver to be metabolically activated.
利用鼠伤寒沙门氏菌TA98检测了小鼠肠道内容物酸性成分中的致突变性。据报道，由于喂食含15%山梨酸的饮食，这些小鼠肝癌发病率很高（石泽等人，1980年）。此外，在为期3个月的喂食期后，15%山梨酸组小鼠肝脏中的谷胱甘肽水平降至对照组的40%，并且这种低水平在很长一段时间（12个月）内一直维持到实验结束。肝谷胱甘肽水平的降低程度与饮食中添加的山梨酸浓度之间也存在密切相关性。因此，喂食15%山梨酸饮食的小鼠所发生的肝癌被认为是长期肝谷胱甘肽水平耗竭以及肠道中各种诱变剂逐渐产生并被吸收转移至肝脏进行代谢激活共同作用的结果。
