Gowrishankar J, Pittard J
J Bacteriol. 1982 Oct;152(1):1-6. doi: 10.1128/jb.152.1.1-6.1982.
The regulator gene pheR, which in Escherichia coli controls the expression of pheA, the structural gene for chorismate mutase P-prephenate dehydratase, was cloned on to multicopy plasmids directly from the E. coli chromosome; this was achieved with the aid of the tetracycline resistance transposon, Tn10, that had been inserted very close to the pheR gene. Subsequently, pheR was subcloned on a 1.1-kilobase-pair fragment on the plasmid vector pBR322; its position on the plasmid was localized by the method of gamma delta-mediated transpositional inactivation. The pheR gene product was identified in maxicells and found to be a protein of subunit molecular weight 19,000, suggesting that the coding segment of the gene is about 500 nucleotide pairs long.
调节基因pheR在大肠杆菌中控制分支酸变位酶P-预苯酸脱水酶的结构基因pheA的表达，该基因通过四环素抗性转座子Tn10直接从大肠杆菌染色体克隆到多拷贝质粒上，Tn10插入位置非常靠近pheR基因。随后，pheR被亚克隆到质粒载体pBR322上一个1.1千碱基对的片段上；通过γδ介导的转座失活方法确定了其在质粒上的位置。在大细胞中鉴定出pheR基因产物，发现它是一种亚基分子量为19000的蛋白质，这表明该基因的编码区段约为500个核苷酸对长。
