Pittard J, Praszkier J, Certoma A, Eggertsson G, Gowrishankar J, Narasaiah G, Whipp M J
Department of Microbiology, University of Melbourne, Parkville, Victoria Australia.
J Bacteriol. 1990 Oct;172(10):6077-83. doi: 10.1128/jb.172.10.6077-6083.1990.
pheV, one of the genes that code for tRNA(Phe), was deleted from the chromosome of a strain of Escherichia coli K-12. As a consequence of this mutation, expression of pheA, the gene for chorismate mutase P-prephenate dehydratase, the first enzyme in the terminal pathway of phenylalanine biosynthesis, was derepressed. Similar derepression of pheA has been reported in pheR mutants of E. coli K-12 (J. Gowrishankar and J. Pittard, J. Bacteriol. 150:1130-1137, 1982). Attempts to introduce a pheR mutation into the delta pheV strain failed under circumstances suggesting that this combination of mutations is lethal. Southern blot analysis of pheV+ and delta pheV strains indicated that there are only two tRNA(Phe) genes in E. coli. It is recommended that the names pheU and pheV be retained for these genes.
pheV是编码tRNA(Phe)的基因之一，它从大肠杆菌K-12菌株的染色体中被删除。作为这种突变的结果，苯丙氨酸生物合成终末途径中的第一种酶——分支酸变位酶P-预苯酸脱水酶的基因pheA的表达被解除阻遏。在大肠杆菌K-12的pheR突变体中也报道了类似的pheA解除阻遏现象（J. Gowrishankar和J. Pittard，《细菌学杂志》150:1130 - 1137，1982年）。在某些情况下，试图将pheR突变引入ΔpheV菌株的尝试失败了，这表明这种突变组合是致死的。对pheV⁺和ΔpheV菌株的Southern印迹分析表明，大肠杆菌中只有两个tRNA(Phe)基因。建议保留pheU和pheV这两个基因的名称。
