Fontes C F, Ricci L C, Oliveira M S, Gatti M S, Pestana de Castro A F
Med Microbiol Immunol. 1982;171(1):43-51. doi: 10.1007/BF02122706.
The production of thermolabile (LT) enterotoxin was compared in a defined medium reported by Staples et al. (SAG medium) for the production of thermostable (ST) enterotoxin and the Casamino acids-Yeast extract (CAYE) medium. Aliquots were drawn frum cultures of an enterotoxigenic (LT+, ST+) E. coli in both media at different times, growth curves were plotted, and culture filtrates tested for toxin activity. Levels of LT and ST and in the SAG showed that it is as suitable as CAYE for the production of LT. The addition of either glucose (1%) or lincomycin (90 microgram/ml) to SAG medium increased LT levels, but no synergistic effort could be observed if both substances were added concomitantly. Cultures in SAG medium incubated stationarily for 72 h at 37 degrees C yielded more LT than shaking cultures incubated similarly.
在斯台普斯等人报道的用于产热稳定（ST）肠毒素的限定培养基（SAG培养基）和酪蛋白氨基酸 - 酵母提取物（CAYE）培养基中，对不耐热（LT）肠毒素的产生进行了比较。在不同时间从两种培养基中的产肠毒素（LT +，ST +）大肠杆菌培养物中取出等分试样，绘制生长曲线，并对培养滤液进行毒素活性测试。SAG中LT和ST的水平表明，它与CAYE一样适合用于产LT。向SAG培养基中添加葡萄糖（1%）或林可霉素（90微克/毫升）可提高LT水平，但如果同时添加这两种物质，则未观察到协同作用。在37℃下静止培养72小时的SAG培养基中的培养物比类似培养的振荡培养物产生更多的LT。
