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Improvements in the passive immune hemolysis test for assaying enterotoxigenic Escherichia coli.用于检测产肠毒素大肠杆菌的被动免疫溶血试验的改进。
J Clin Microbiol. 1980 Nov;12(5):714-7. doi: 10.1128/jcm.12.5.714-717.1980.
2
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4
Value of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.被动免疫溶血法在检测产肠毒素大肠杆菌产生的热不稳定肠毒素中的价值
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5
Storage of Escherichia coli thermolabile enterotoxin on filter papers for assay by immune haemolysis reactions.将大肠杆菌不耐热肠毒素储存在滤纸上，用于通过免疫溶血反应进行检测。
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Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture.利用组织培养中的肾上腺细胞检测不耐热大肠杆菌肠毒素。
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Mortality of older acutely admitted medical patients after early discharge from emergency departments: a nationwide cohort study.老年急症入院患者在急诊科提前出院后的死亡率：一项全国性队列研究。
BMC Geriatr. 2021 Jul 2;21(1):410. doi: 10.1186/s12877-021-02355-y.
2
Single radial immune hemolysis test for detection of Escherichia coli thermolabile enterotoxin.用于检测大肠杆菌不耐热肠毒素的单向放射免疫溶血试验。
J Clin Microbiol. 1981 Nov;14(5):473-8. doi: 10.1128/jcm.14.5.473-478.1981.
3
Detection of heat-labile (LT) enterotoxin of enterotoxigenic Escherichia coli by the radial immune hemolysis test: a modification for clinical use.用放射免疫溶血试验检测产肠毒素大肠杆菌的不耐热（LT）肠毒素：临床应用的一种改进方法
Med Microbiol Immunol. 1982;171(3):171-8. doi: 10.1007/BF02123625.
4
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Med Microbiol Immunol. 1982;171(1):43-51. doi: 10.1007/BF02122706.
5
Storage of Escherichia coli thermolabile enterotoxin on filter papers for assay by immune haemolysis reactions.将大肠杆菌不耐热肠毒素储存在滤纸上，用于通过免疫溶血反应进行检测。
Med Microbiol Immunol. 1984;172(4):243-8. doi: 10.1007/BF02123719.
6
Indirect haemagglutination test for the detection of thermolabile (LT) enterotoxin from Escherichia coli.用于检测大肠杆菌不耐热（LT）肠毒素的间接血凝试验。
Med Microbiol Immunol. 1986;175(4):251-60. doi: 10.1007/BF02123733.
7
Stability of thermolabile (LT) enterotoxin produced from enterotoxigenic Escherichia coli strains maintained in vitro.
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Polymyxin B-Induced Release of Low-Molecular-Weight, Heat-Labile Enterotoxin from Escherichia coli.多黏菌素 B 诱导大肠埃希菌释放低分子量热不稳定肠毒素。
Infect Immun. 1974 Nov;10(5):1010-7. doi: 10.1128/iai.10.5.1010-1017.1974.
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A quantitative complement fixation test: titration of luetic sera by the unit of 50 per cent hemolysis.定量补体结合试验：以50%溶血单位滴定梅毒血清。
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Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture.利用组织培养中的肾上腺细胞检测不耐热大肠杆菌肠毒素。
Science. 1974 Jan 25;183(4122):334-6. doi: 10.1126/science.183.4122.334.
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Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.环磷酸腺苷与中国仓鼠卵巢细胞形态改变：一种用于检测霍乱弧菌和大肠杆菌肠毒素的快速、灵敏的体外测定法。
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Assay of Escherichia coli heat-labile enterotoxin with vero cells.用非洲绿猴肾细胞检测大肠杆菌不耐热肠毒素
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Direct serological assay for the heat-labile enterotoxin of Escherichia coli, using passive immune hemolysis.采用被动免疫溶血法对大肠杆菌不耐热肠毒素进行直接血清学检测。
Infect Immun. 1977 May;16(2):604-9. doi: 10.1128/iai.16.2.604-609.1977.
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Factors influencing heat-labile Escherichia coli enterotoxin activity.影响不耐热大肠杆菌肠毒素活性的因素。
Infect Immun. 1976 Aug;14(2):383-8. doi: 10.1128/iai.14.2.383-388.1976.

用于检测产肠毒素大肠杆菌的被动免疫溶血试验的改进。

Improvements in the passive immune hemolysis test for assaying enterotoxigenic Escherichia coli.

作者信息

de Castro A F, Serafim M B, Gomes J A, Gatti M S

出版信息

J Clin Microbiol. 1980 Nov;12(5):714-7. doi: 10.1128/jcm.12.5.714-717.1980.

DOI:10.1128/jcm.12.5.714-717.1980
PMID:6792218
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273679/
Abstract

The sensitivity of the passive immune hemolysis test for the detection of heat-labile enterotoxin from Escherichia coli was increased when the test was carried out with Veronal-buffered saline plus Ca2+ and Mg2+ as diluent, instead of phosphate-buffered saline. The performance of the test was further improved by using stationary cultures to which mitomycin C had been added at the end of the lag phase.

摘要

当用含有Ca2+和Mg2+的巴比妥缓冲盐水作为稀释剂，而不是磷酸盐缓冲盐水进行被动免疫溶血试验来检测大肠杆菌热不稳定肠毒素时，该试验的灵敏度提高了。通过使用在延迟期末添加了丝裂霉素C的静止培养物，该试验的性能得到了进一步改善。