用于检测产肠毒素大肠杆菌的被动免疫溶血试验的改进。
Improvements in the passive immune hemolysis test for assaying enterotoxigenic Escherichia coli.
作者信息
de Castro A F, Serafim M B, Gomes J A, Gatti M S
出版信息
J Clin Microbiol. 1980 Nov;12(5):714-7. doi: 10.1128/jcm.12.5.714-717.1980.
Abstract
The sensitivity of the passive immune hemolysis test for the detection of heat-labile enterotoxin from Escherichia coli was increased when the test was carried out with Veronal-buffered saline plus Ca2+ and Mg2+ as diluent, instead of phosphate-buffered saline. The performance of the test was further improved by using stationary cultures to which mitomycin C had been added at the end of the lag phase.
摘要
当用含有Ca2+和Mg2+的巴比妥缓冲盐水作为稀释剂，而不是磷酸盐缓冲盐水进行被动免疫溶血试验来检测大肠杆菌热不稳定肠毒素时，该试验的灵敏度提高了。通过使用在延迟期末添加了丝裂霉素C的静止培养物，该试验的性能得到了进一步改善。
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