Rodwell A W
Rev Infect Dis. 1982 May-Jun;4 Suppl:S8-17. doi: 10.1093/clinids/4.supplement_1.s8.
The proteins of 91 strains of Mycoplasma from 19 established species and three groups of uncertain taxonomic position were separated by two-dimensional polyacrylamide gel electrophoresis. Considerable variations in protein patterns were found among strains from the same species or subspecies; the percentage of matching spots (% of congruence) ranged from 42%-100%, but many proteins were strongly conserved in all strains of a given species. Maps of these conserved proteins could be used to identify strains. It was concluded that (1) large colony-type strains of Mycoplasma mycoides subspecies mycoides are more closely related to M. mycoides subspecies capri than to small colony-type strains of M. mycoides subspecies mycoides; (2) Mycoplasma capricolum, Leach group 7, and the F38 group of mycoplasmas are all related to M. mycoides; (3) the 2D group of strains are not related to either M. mycoides or Mycoplasma bovis, (4) M. bovis and Mycoplasma agalactiae are closely related; (5) apart from a small overlap in pattern between Acholeplasma laidlawii and Acholeplasma granularum, the type strains of seven established species of Acholeplasma each had distinct protein patterns that justified their classification as separate species; and (6) analysis of two-dimensional protein patterns is a valuable method for resolving problems in mycoplasma taxonomy and for identifying strains.
采用双向聚丙烯酰胺凝胶电泳法对来自19个已确定种和3个分类地位不确定组的91株支原体的蛋白质进行了分离。在同一物种或亚种的菌株之间发现了蛋白质图谱的显著差异；匹配点的百分比（一致性百分比）在42%-100%之间，但许多蛋白质在给定物种的所有菌株中都高度保守。这些保守蛋白质的图谱可用于鉴定菌株。得出的结论是：（1）丝状支原体丝状亚种的大菌落型菌株与丝状支原体山羊亚种的亲缘关系比与丝状支原体丝状亚种的小菌落型菌株更近；（2）山羊支原体、利奇第7组和支原体F38组均与丝状支原体有关；（3）2D组菌株与丝状支原体或牛支原体均无关系；（4）牛支原体和无乳支原体亲缘关系密切；（5）除莱氏无胆甾原体和颗粒无胆甾原体之间的图谱有小部分重叠外，7个已确定的无胆甾原体种的模式菌株各自具有独特的蛋白质图谱，这证明它们可分类为不同的种；（6）二维蛋白质图谱分析是解决支原体分类学问题和鉴定菌株的一种有价值的方法。
