Aydin G, Okiye S E, Zincke H
J Urol. 1982 Dec;128(6):1401-3. doi: 10.1016/s0022-5347(17)53517-x.
The effect of 35 minutes of warm ischemia (37C) on renal function and adenine nucleotide content of canine kidneys preserved for 24 and 48 hours in Euro-Collins (EC) solution was investigated. In addition, the effect of donor pretreatment with intravenous mannitol, furosemide and methylprednisolone and the addition of adenosine triphosphate (ATP/MgCl2) to the EC flush and storage solution was studied. Donor pretreatment or the addition of ATP/MgCl2 to the flushing and storage solution did not significantly affect postautotransplant renal function of kidneys stored for 24 hours, although it improved tissue adenine nucleotide levels. Results after 48-hour preservation were significantly poorer. These experiments demonstrate that canine kidneys subjected to 35 minutes of warm ischemia time can be stored for 24 hours in EC solution and thereafter provide immediate life-sustaining function.
研究了35分钟热缺血（37℃）对在欧洲柯林斯（EC）溶液中保存24小时和48小时的犬肾肾功能及腺嘌呤核苷酸含量的影响。此外，还研究了供体静脉注射甘露醇、呋塞米和甲基泼尼松龙预处理以及在EC冲洗液和保存液中添加三磷酸腺苷（ATP/MgCl₂）的效果。供体预处理或在冲洗液和保存液中添加ATP/MgCl₂对保存24小时的肾脏自体移植后肾功能没有显著影响，尽管它提高了组织腺嘌呤核苷酸水平。48小时保存后的结果明显较差。这些实验表明，经历35分钟热缺血时间的犬肾可以在EC溶液中保存24小时，然后提供即时维持生命的功能。
