Garvin P J, Jellinek M, Morgan R, Codd J E
Arch Surg. 1981 Feb;116(2):221-4. doi: 10.1001/archsurg.1981.01380140067015.
The metabolic consequences of low-temperature kidney preservation by pulsatile perfusion and cold storage were evaluated in canine kidneys by serial adenosine triphosphate (ATP) determinations. Serial cortical ATP levels were determined (1) after optimal harvest, (2) after 60 minutes of warm ischemia, and (3) after 60 minutes of warm ischemia followed by an intra-aortic infusion of ATP-MgCl2. After 24 hours of preservation, cortical ATP levels were significantly greater when pulsatile perfusion was used-both after optimal harvest and after 60 minutes of warm ischemia. Cortical ATP levels were significantly greater at 24 hours in both perfused and cold-storage kidneys when an intra-aortic infusion of ATP-MgCl2 was used following ischemia. These results may explain the 24-hour limitation of cold storage as well as its inferior results following ischemia injury. Furthermore, since ATP is critical in cellular metabolism, the technique of intra-aortic ATP-MgCl2 infusion warrants further investigation in the field of organ preservation.
通过连续测定犬肾中的三磷酸腺苷（ATP），评估了搏动灌注和冷藏低温保存肾脏的代谢后果。连续测定皮质ATP水平：（1）在最佳摘取后，（2）在60分钟热缺血后，以及（3）在60分钟热缺血后经主动脉内输注ATP - MgCl2。保存24小时后，无论是在最佳摘取后还是在60分钟热缺血后，使用搏动灌注时皮质ATP水平均显著更高。当缺血后经主动脉内输注ATP - MgCl2时，灌注和冷藏的肾脏在24小时时皮质ATP水平均显著更高。这些结果可能解释了冷藏24小时的局限性以及缺血损伤后冷藏效果较差的原因。此外，由于ATP在细胞代谢中至关重要，主动脉内输注ATP - MgCl2技术值得在器官保存领域进一步研究。
