Foreman R C, Judah J D
Biosci Rep. 1982 Dec;2(12):995-1002. doi: 10.1007/BF01122167.
When membranes from Escherichia coli MRE 600 are added to an in vitro translation system, they are able to cleave correctly prehuman placental lactogen to yield the mature hormone. The protein was identified by SDS/polyacrylamide-gel electrophoresis and by determining its amino acid sequence. These studies were aided by the development of a new method for the separation of human placental lactogen from its precursor.
当将来自大肠杆菌MRE 600的膜添加到体外翻译系统中时，它们能够正确切割人前胎盘催乳素前体，以产生成熟的激素。通过SDS/聚丙烯酰胺凝胶电泳和确定其氨基酸序列对该蛋白质进行了鉴定。一种从人前胎盘催乳素前体中分离它的新方法的开发辅助了这些研究。
