Wilkinson P C, Haston W S, Shields J M
Clin Exp Immunol. 1982 Dec;50(3):461-73.
In this review, we discuss some physical and chemical determinants of locomotor behaviour of phagocytic cells and lymphocytes as studied in visual assays, paying particular attention to the following points. (a) A distinction is made between chemokinesis and other forms of kinesis. We propose that the term chemokinesis be reserved for responses resulting from selective recognition of chemical substances. Many kineses in leucocytes may not involve such recognition, but may result from a variety of physical factors, such as those that alter the adhesiveness between cell and substratum. (b) Neutrophils moving in aligned gels of collagen or fibrin show contact guidance of locomotion, i.e. bidirectional movement along the axis of alignment of the fibres of the gel. Thus neutrophils show directional locomotion not only in chemotactic gradients but also in response to physical properties of the substratum. (c) Lymphocytes adhere poorly and move poorly on 2D protein coated substrata. However they move rapidly through 3D collagen gels. This locomotion may be independent of adhesion since lymphocytes may gain traction for locomotion by expanding pseudopods into gaps in the gel matrix, then using the pseudopod as an anchor for subsequent locomotion in any direction.
在本综述中，我们讨论了在视觉分析中研究的吞噬细胞和淋巴细胞运动行为的一些物理和化学决定因素，特别关注以下几点。（a）趋化性与其他形式的运动性之间存在区别。我们建议将趋化性一词保留用于由对化学物质的选择性识别所产生的反应。白细胞中的许多运动可能不涉及这种识别，而是可能由多种物理因素引起，例如那些改变细胞与基质之间粘附性的因素。（b）在排列的胶原蛋白或纤维蛋白凝胶中移动的中性粒细胞表现出运动的接触导向，即沿着凝胶纤维排列轴的双向运动。因此，中性粒细胞不仅在趋化梯度中表现出定向运动，而且对基质的物理性质也有反应。（c）淋巴细胞在二维蛋白质包被的基质上粘附性差且移动性差。然而，它们能快速穿过三维胶原蛋白凝胶。这种运动可能与粘附无关，因为淋巴细胞可能通过将伪足扩展到凝胶基质的间隙中获得运动牵引力，然后将伪足用作后续向任何方向运动的锚点。
