Jones E W, Zubenko G S, Parker R R
Genetics. 1982 Dec;102(4):665-77. doi: 10.1093/genetics/102.4.665.
The pep4-3 mutation results in a 90-95% reduction in the levels of five vacuolar hydrolases in yeast, including proteinases A and B, carboxypeptidase Y, RNase(s) and the repressible alkaline phosphatase. The mutation is without effect on two secreted glycoproteins, on an enzyme of the vacuolar membrane, and on a proteinase located outside of the vacuole. Mutations at the PEP4 locus exhibit a dosage effect on the levels of some, but not all, of the enzymes whose expression requires the function of the gene.
pep4-3突变导致酵母中五种液泡水解酶的水平降低90-95%，这些酶包括蛋白酶A和B、羧肽酶Y、核糖核酸酶和可阻遏的碱性磷酸酶。该突变对两种分泌糖蛋白、液泡膜的一种酶以及液泡外的一种蛋白酶没有影响。PEP4基因座的突变对某些（但不是全部）其表达需要该基因功能的酶的水平表现出剂量效应。
