Rosett W, Hodges G R
J Clin Microbiol. 1980 Jan;11(1):30-4. doi: 10.1128/jcm.11.1.30-34.1980.
Single clinical isolates of eight species of microorganisms were incubated in solutions of heparin and brain heart infusion broth at various concentrations to determine the possible antibacterial effect of heparin. At heparin concentrations ranging from 12.5 to 400 U/ml, the effect on Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Staphylococcus aureus, and S. epidermidis varied with brain heart infusion broth concentrations of 1.2 to 10% and inocula of 10(2) to 10(6) colony-forming units per ml; similar effects were not observed with Klebsiella pneumoniae, Enterobacter aerogenes, and Citrobacter spp. The minimal inhibitory concentrations of heparin for ten strains of each species were determined in 2.5% brain heart infusion broth with inocula of 10(4) colony-forming units per ml. All 10 isolates of S. aureus and all 10 of S. epidermidis were inhibited by heparin concentrations of 125 to 500 U/ml. Three E. coli, four P. aeruginosa, and nine C. albicans strains were inhibited by </=500 U of heparin per ml. None of the K. pneumoniae, E. aerogenes, Enterobacter cloacae, and Citrobacter spp. was inhibited by heparin at </=500 U/ml. Heparin inhibition of S. aureus in 2.5% brain heart infusion broth-500 U of heparin per ml could be quantitatively overcome by addition of magnesium, calcium, or magnesium and calcium. These data suggest that the growth of microorganisms from heparin-containing material may be suppressed.
将八种微生物的单个临床分离株在不同浓度的肝素溶液和脑心浸液肉汤中培养，以确定肝素可能的抗菌作用。在肝素浓度为12.5至400 U/ml的范围内，其对大肠杆菌、铜绿假单胞菌、白色念珠菌、金黄色葡萄球菌和表皮葡萄球菌的作用随脑心浸液肉汤浓度为1.2%至10%以及接种量为每毫升10²至10⁶个菌落形成单位而变化；肺炎克雷伯菌、产气肠杆菌和柠檬酸杆菌属未观察到类似作用。在含2.5%脑心浸液肉汤、接种量为每毫升10⁴个菌落形成单位的条件下，测定了每种菌株的10个菌株对肝素的最低抑菌浓度。所有10株金黄色葡萄球菌和所有10株表皮葡萄球菌均被125至500 U/ml的肝素浓度所抑制。三株大肠杆菌、四株铜绿假单胞菌和九株白色念珠菌菌株被每毫升≤500 U的肝素所抑制。肺炎克雷伯菌、产气肠杆菌、阴沟肠杆菌和柠檬酸杆菌属在≤500 U/ml的肝素浓度下均未被抑制。在含2.5%脑心浸液肉汤-每毫升500 U肝素中，肝素对金黄色葡萄球菌的抑制作用可通过添加镁、钙或镁和钙来定量克服。这些数据表明，含肝素材料中微生物的生长可能会受到抑制。
