人肝脏针吸活检标本微粒体组分中的单加氧酶活性
Monooxygenase activity of human liver in microsomal fractions of needle biopsy specimens.
作者信息
Boobis A R, Brodie M J, Kahn G C, Fletcher D R, Saunders J H, Davies D S
出版信息
Br J Clin Pharmacol. 1980 Jan;9(1):11-9. doi: 10.1111/j.1365-2125.1980.tb04790.x.
Abstract
1 Methods are described for the determination of mixed function oxidase activity in microsomal fractions from percutaneous needle biopsies of human liver. 2 Activities of needle biopsy samples were comparable with those of wedge biopsy sample obtained at laparotomy from different subjects. 3 Although cytochrome P-450 content of liver from rat and man was similar, human AHH activity was only 10% of that in the rat. 4 In biopsies with preserved hepatic architecture, AHH activity and cytochrome P-450 content showed a significant positive correlation. 5 Cigarette smoking significantly increased both AHH activity and turnover number, but not cytochrome P-450 content, of biopsies with normal architecture. 6 The presence of liver disease caused a significant decrease in AHH activity and cytochrome P-450 content.
摘要
- 本文描述了测定人肝脏经皮针刺活检微粒体组分中混合功能氧化酶活性的方法。2. 针刺活检样本的活性与不同受试者剖腹术获取的楔形活检样本的活性相当。3. 虽然大鼠和人的肝脏细胞色素P - 450含量相似，但人芳烃羟化酶（AHH）活性仅为大鼠的10%。4. 在肝组织结构保存完好的活检样本中，AHH活性与细胞色素P - 450含量呈显著正相关。5. 吸烟显著增加了结构正常的活检样本的AHH活性和转换数，但未增加细胞色素P - 450含量。6. 肝脏疾病的存在导致AHH活性和细胞色素P - 450含量显著降低。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Detoxication of drugs and other foreign compounds by liver microsomes.肝脏微粒体对药物及其他外来化合物的解毒作用。
Science. 1955 Apr 22;121(3147):603-4. doi: 10.1126/science.121.3147.603.
3
THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.肝微粒体的一氧化碳结合色素。I. 其血红蛋白性质的证据。
J Biol Chem. 1964 Jul;239:2370-8.
4
Mechanism of hepatic drug oxidation and its relationship to individual differences in rates of oxidation in man.肝脏药物氧化机制及其与人氧化速率个体差异的关系。
Ann N Y Acad Sci. 1971 Jul 6;179:411-20. doi: 10.1111/j.1749-6632.1971.tb46918.x.
5
In vitro inhibition of rat and human liver microsomal enzymes by 4-hydroxycoumarin anticoagulants and related compounds.4-羟基香豆素类抗凝剂及相关化合物对大鼠和人肝微粒体酶的体外抑制作用。
Chem Biol Interact. 1972 Sep;5(4):255-63. doi: 10.1016/0009-2797(72)90029-4.
6
Acetaminophen-induced hepatic necrosis. II. Role of covalent binding in vivo.对乙酰氨基酚诱导的肝坏死。II. 体内共价结合的作用。
J Pharmacol Exp Ther. 1973 Oct;187(1):195-202.
7
Existence and separation of three forms of cytochrome P-450 from rat liver microsomes.
J Biol Chem. 1973 Jul 25;248(14):4947-55.
8
Some observations on the microsomal electron transport system and activities of drug oxidizing enzymes in human liver.
Chem Pharm Bull (Tokyo). 1973 Jan;21(1):8-11. doi: 10.1248/cpb.21.8.
9
10
[Dealkylation of ethylmorphine and p-C-hydroxylation of aniline in liver microsomes of humans and male and female rats].[人及雄性和雌性大鼠肝脏微粒体中乙基吗啡的脱烷基化及苯胺的对-碳-羟基化作用]
Biochem Pharmacol. 1972 Aug 15;21(16):2169-80. doi: 10.1016/0006-2952(72)90032-9.
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