Hayatdavoudi G, Crapo J D, Miller F J, O'Neil J J
J Appl Physiol Respir Environ Exerc Physiol. 1980 Feb;48(2):389-93. doi: 10.1152/jappl.1980.48.2.389.
The total lung capacity (TLC) of rats was measured in vivo and was compared to the displacement volume of the lungs following intratracheal fixation with glutaraldehyde or formaldehyde solution. When glutaraldehyde was used the speed of infusion of the fixative was an important factor in the final degree of lung inflation achieved. With a low rate of fixative infusion and a final pressure of 20 cm of fixative the glutaraldehyde-fixed lungs inflated to 55% TLC. With a high initial flow of glutaraldehyde and a final pressure of 20 cm of fixative the lungs inflated to 84% TLC. Fixation of lungs inside the intact chest wall was found to result in a higher degree of inflation. With a reservoir height of 20 cm and a low rate of fixative infusion lungs fixed in situ reached 74% TLC, whereas lungs fixed in situ, but from animals that have been exsanguinated prior to fixation, inflated to only 58% TLC. This suggests that the volume of the blood in the lungs prior to infusion of glutaraldehyde influences the degree of inflation achieved. Formaldehyde-fixed lungs required 72 h to be completely fixed and they were inflated to 90% TLC when a reservoir height of 20 cm was used. Because of the slow rate of fixation using with formaldehyde solution the rate of infusion was found not to limit the degree of inflation that could be achieved.
在体内测量大鼠的肺总量（TLC），并将其与用戊二醛或甲醛溶液气管内固定后肺的置换体积进行比较。当使用戊二醛时，固定剂的输注速度是最终实现的肺膨胀程度的一个重要因素。以低固定剂输注速率和20厘米固定剂的最终压力，戊二醛固定的肺膨胀至TLC的55%。以高初始戊二醛流速和20厘米固定剂的最终压力，肺膨胀至TLC的84%。发现在完整胸壁内固定肺会导致更高程度的膨胀。在储液器高度为20厘米且固定剂输注速率较低的情况下，原位固定的肺达到TLC的74%，而原位固定但在固定前已放血的动物的肺仅膨胀至TLC的58%。这表明在注入戊二醛之前肺内的血液量会影响实现的膨胀程度。甲醛固定的肺需要72小时才能完全固定，当使用20厘米的储液器高度时，它们膨胀至TLC的90%。由于使用甲醛溶液固定的速度较慢，发现输注速率不会限制可实现的膨胀程度。
