Cook D I, Revzin A
J Bacteriol. 1980 Mar;141(3):1279-83. doi: 10.1128/jb.141.3.1279-1283.1980.
The catabolite activator protein was assayed in extracts from the minicell-producing Escherichia coli strain P678-54. The level of catabolite activator protein was found to be the same in both parent cells and purified minicells, regardless of whether the bacteria were grown on glucose (which leads to low intracellular cyclic adenosine monophosphate levels) or on glycerol-yeast extract or LB broth (which lead to high cyclic adenosine monophosphate concentrations in the cell). Thus, at any given time most catabolite activator protein molecules are found in the cytoplasm. The implications of this for the mechanism of catabolite activator protein action at catabolite-sensitive operons are discussed.
在产生微小细胞的大肠杆菌菌株P678 - 54的提取物中检测了分解代谢物激活蛋白。结果发现，无论细菌是在葡萄糖（导致细胞内环状单磷酸腺苷水平较低）上生长，还是在甘油 - 酵母提取物或LB肉汤（导致细胞内环状单磷酸腺苷浓度较高）上生长，亲本细胞和纯化的微小细胞中的分解代谢物激活蛋白水平都是相同的。因此，在任何给定时间，大多数分解代谢物激活蛋白分子都存在于细胞质中。本文讨论了这一现象对分解代谢物激活蛋白在分解代谢敏感操纵子上的作用机制的影响。
