Collins M D, Goodfellow M, Minnikin D E
J Gen Microbiol. 1982 Jan;128(1):129-49. doi: 10.1099/00221287-128-1-129.
Acid methanolysates of 104 strains of coryneform and related bacteria were examined for mycolic acids by thin-layer chromatography and mass spectrometry. The mycolic acids of Corynebacterium sensu stricto strains were not uniform in size and structure, and considerable variation existed within the approximate limiting range of C22 to C36. Two species, Corynebacterium bovis and 'Corynebacterium mycetoides', were particularly distinctive; the former had exceptionally low molecular weight mycolic acids (C22 to C32), whereas the latter contained major amounts of mycolates with a side-chain possessing an odd number of carbon atoms. Caseobacter polymorphus contained mycolic acids of a similar size (C30 to C36) to those of true corynebacteria. The mycolates of organisms assigned to the genus Rhodococcus were generally larger (C30 to C56), although a clear distinction between two corynebacteria and rhodococci cannot presently be made by analysis of mycolic acids alone. Strains labelled 'Arthrobacter roseoparaffinus', Corynebacterium equi and Corynebacterium hoagii contained mycolic esters with molecular weights 'intermediate' in size between those of true corynebacteria and rhodococci. The mycolic acid data correlate well with other major trends in coryneform taxonomy and support earlier suggestions that they are of value in the classification of the group.
通过薄层色谱法和质谱分析法，对104株棒状杆菌及相关细菌的酸性甲醇裂解产物进行了分枝菌酸检测。严格意义上的棒状杆菌菌株的分枝菌酸在大小和结构上并不统一，在大约C22至C36的限定范围内存在相当大的差异。牛棒状杆菌和“类真菌棒状杆菌”这两个物种尤为独特；前者具有分子量极低的分枝菌酸（C22至C32），而后者含有大量带有奇数个碳原子侧链的分枝菌酸盐。多形丙酸杆菌所含分枝菌酸的大小（C30至C36）与真正的棒状杆菌类似。尽管目前仅通过分枝菌酸分析无法明确区分棒状杆菌和红球菌，但归入红球菌属的生物体的分枝菌酸盐通常更大（C30至C56）。标记为“玫瑰石蜡节杆菌”、马棒状杆菌和霍氏棒状杆菌的菌株含有分子量在真正棒状杆菌和红球菌之间“中等”大小的分枝菌酸酯。分枝菌酸数据与棒状杆菌分类学中的其他主要趋势密切相关，并支持了它们在该类群分类中具有价值的早期观点。
