99m锝标记的人白细胞。一项体外功能研究。

99mTc labelled human leukocytes. An in vitro functional study.

作者信息

Linhart N, Bok B, Gougerot M, Gaillard M T, Meignan M

出版信息

Acta Haematol. 1980;63(2):71-80. doi: 10.1159/000207374.

DOI:10.1159/000207374

Abstract

A new method of human leukocyte labelling is described. 20 ml of human blood are allowed to sediment in a dextran solution and the remaining red blood cells are removed using NH4C1. The leukocyte-rich suspension is incubated with a Sn-pyrophosphate solution, and then labelled, using 20 mCi 99mTc pertechnetate. Labelling efficiency is about 30%. Functional activity was shown to be satisfactory: (1) appearance under electron microscopy was quite normal; (2) Klebsiella ingestion, zymosan-stimulated oxygen consumption and iodination were similar in labelled and unlabelled cells, and (3) chemotaxis revealed no alteration in cell migration.

摘要

描述了一种新的人类白细胞标记方法。将20毫升人体血液置于葡聚糖溶液中使其沉降，然后用氯化铵去除剩余的红细胞。将富含白细胞的悬浮液与焦磷酸锡溶液孵育，然后用20毫居里的高锝酸盐99mTc进行标记。标记效率约为30%。功能活性显示令人满意：(1)电子显微镜下外观相当正常；(2)标记和未标记细胞对克雷伯菌的摄取、酵母聚糖刺激的氧消耗和碘化相似，以及(3)趋化性显示细胞迁移无改变。