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纤毛亚纲腹毛目纤毛虫贻贝棘尾虫中的染色质消除

Chromatin elimination in the hypotrichous ciliate Stylonychia mytilus.

作者信息

Meyer G F, Lipps H J

出版信息

Chromosoma. 1980;77(3):285-97. doi: 10.1007/BF00286054.

DOI:10.1007/BF00286054
PMID:6768532
Abstract

Chromatin elimination in the hypotrichous ciliate Stylonychia mytilus was studied by means of electron microscopy using a microspreading procedure. In the polytene chromosomes of the macronuclear anlagen three organization patterns are observed: Bands of various size composed of 300 A chromatin fibers, large blocks of 300 A nucleofilaments which probably represent the "heterochromatic" regions of the chromosome and axial 120 A filaments. Those DNA sequences which become eliminated belong to the 300 A fiber type. The eliminated chromatin occurs in the form of rings of variable size corresponding to a DNA content between 18 and 160 Kb while the axial 120 A filaments appear to be preserved.

摘要

利用微铺展技术,通过电子显微镜对贻贝棘尾虫(Stylonychia mytilus)这种腹毛目纤毛虫中的染色质消除现象进行了研究。在大核原基的多线染色体中观察到三种组织模式:由300埃染色质纤维组成的各种大小的带、可能代表染色体“异染色质”区域的300埃核丝大区域以及轴向120埃细丝。那些被消除的DNA序列属于300埃纤维类型。被消除的染色质以大小可变的环的形式出现,其DNA含量在18至160千碱基之间,而轴向120埃细丝似乎得以保留。

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