Takeda K, Tanaka K
Antonie Van Leeuwenhoek. 1980;46(1):15-25. doi: 10.1007/BF00422225.
The development of intracytoplasmic membranes of Methanomonas margaritae cells grown under different culture conditions was studied. Growth on methane was strongly accelerated by the addition of copper ions. Acceleration by copper, however, was not observed in the case of growth on methanol. Cells grown on methane with copper possessed intracytoplasmic membranes along the cell periphery. When the the organism was grown in a medium lacking copper, intracytoplasmic membranes appeared as large vesicles surrounded by a unit membrane at the periphery of the cell. The vesicles originated from paired membranes due to the absence of copper in the medium. Cells grown on methanol with or without copper possessed a number of vesicles of different sizes arranged in a chain along the cell periphery. The possible relationship between membrane arrangement and methane oxidation is discussed.
研究了在不同培养条件下生长的玛格丽特甲烷单胞菌细胞胞内膜的发育情况。添加铜离子可显著促进甲烷上的生长。然而，在甲醇上生长时未观察到铜的促进作用。在有铜的情况下以甲烷为生长底物的细胞，其胞内膜沿细胞周边分布。当该生物体在缺乏铜的培养基中生长时，胞内膜呈现为在细胞周边被单位膜包围的大囊泡。由于培养基中缺乏铜，这些囊泡起源于成对的膜。在有或没有铜的情况下以甲醇为生长底物的细胞，沿细胞周边有许多不同大小的囊泡呈链状排列。文中讨论了膜排列与甲烷氧化之间可能的关系。
