根皮苷和硫醇试剂对泌乳大鼠乳腺高尔基体膜囊泡半乳糖基转移酶活性的影响。
Effects of phlorrhizin and thiol reagents on the galactosyltransferase activity of Golgi membrane vesicles of lactating rat mammary gland.
作者信息
Kuhn N J, White A
出版信息
Biochem J. 1980 May 15;188(2):503-7. doi: 10.1042/bj1880503.
Abstract
- The ability of phlorrhizin to inhibit the galactosylation of glucose was re-examined with Golgi membrane vesicles purified from rat mammary gland, and extended to the galactosylation of several glucose analogues and N-acylglucosamines. 2. The inhibition is ascribed, contrary to previous conclusions, to a general annealing of leaky membranes comprising a minority of the vesicles. 3. Three thiol reagents were able to inhibit the galactosylation of N-acylglucosamines with less, or no, inhibition of galactosylation of glucose. This demonstrates the existence of a Golgi membrane carrier that distinguishes between glucose and N-acylglucosamines.
摘要
- 利用从大鼠乳腺中纯化得到的高尔基体膜囊泡，重新研究了根皮苷抑制葡萄糖半乳糖基化的能力，并将其扩展至几种葡萄糖类似物和N-酰基葡糖胺的半乳糖基化研究。2. 与之前的结论相反，这种抑制作用归因于由少数囊泡组成的渗漏膜的普遍退火。3. 三种硫醇试剂能够抑制N-酰基葡糖胺的半乳糖基化，而对葡萄糖的半乳糖基化抑制较少或无抑制作用。这证明了存在一种能区分葡萄糖和N-酰基葡糖胺的高尔基体膜载体。
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引用本文的文献
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本文引用的文献
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Eur J Biochem. 1980 Jan;103(2):377-85. doi: 10.1111/j.1432-1033.1980.tb04324.x.
2
The subcellular distribution of the A and B proteins of lactose synthetase in bovine and rat mammary tissue.乳糖合成酶A和B蛋白在牛和大鼠乳腺组织中的亚细胞分布。
J Biol Chem. 1966 Dec 10;241(23):5526-32.
3
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Galactosyltransferase acceptor specificity of the lactose synthetase A protein.乳糖合成酶A蛋白的半乳糖基转移酶受体特异性
J Biol Chem. 1970 Oct 10;245(19):5057-61.
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