Vick B A, Feng P, Zimmerman D C
Lipids. 1980 Jun;15(6):468-71. doi: 10.1007/BF02534074.
13-[18-O]Hydroperoxylinolenic acid was permitted to react with an extract of flaxseed acetone powder containing hydroperoxide cyclase activity. The resulting product, 12-oxo-cis-10,cis-15-phytodienoic acid (12-oxo-PDA), contained 18O in the carbonyl oxygen at carbon 12, suggesting that an epoxide was an intermediate in the hyderoperoxide cyclase reaction. A substrate specificity study showed that a cis double bond beta, gamma to the conjugated hydroperoxide group was essential for the substrate to be converted to a cyclic product by hydroperoxide cyclase.
使13-[18-O]氢过氧化亚麻酸与含有氢过氧化物环化酶活性的亚麻籽丙酮粉提取物反应。所得产物12-氧代-顺式-10,顺式-15-植物二烯酸（12-氧代-PDA）在碳12的羰基氧中含有18O，这表明环氧化物是氢过氧化物环化酶反应的中间体。一项底物特异性研究表明，共轭氢过氧化物基团β、γ位的顺式双键对于底物被氢过氧化物环化酶转化为环状产物至关重要。
