Laboratory of Disease Genomics and Individualized Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
Division of Hematology, Department of Medicine, University of Washington, Seattle, WA 98195, USA.
Genomics. 2013 Nov-Dec;102(5-6):431-441. doi: 10.1016/j.ygeno.2013.09.005. Epub 2013 Oct 8.
To explore the mechanisms controlling erythroid differentiation and development, we analyzed the genome-wide transcription dynamics occurring during the differentiation of human embryonic stem cells (HESCs) into the erythroid lineage and development of embryonic to adult erythropoiesis using high throughput sequencing technology. HESCs and erythroid cells at three developmental stages: ESER (embryonic), FLER (fetal), and PBER (adult) were analyzed. Our findings revealed that the number of expressed genes decreased during differentiation, whereas the total expression intensity increased. At each of the three transitions (HESCs-ESERs, ESERs-FLERs, and FLERs-PBERs), many differentially expressed genes were observed, which were involved in maintaining pluripotency, early erythroid specification, rapid cell growth, and cell-cell adhesion and interaction. We also discovered dynamic networks and their central nodes in each transition. Our study provides a fundamental basis for further investigation of erythroid differentiation and development, and has implications in using ESERs for transfusion product in clinical settings.
为了探索控制红细胞分化和发育的机制，我们使用高通量测序技术分析了人类胚胎干细胞（HESCs）向红细胞谱系分化和胚胎到成人红细胞生成过程中发生的全基因组转录动态。分析了三个发育阶段的 HESCs 和红细胞：ESER（胚胎）、FLER（胎儿）和 PBER（成人）。我们的研究结果表明，分化过程中表达基因的数量减少，而总表达强度增加。在三个转变中的每一个（HESCs-ESERs、ESERs-FLERs 和 FLERs-PBERs），观察到许多差异表达的基因，这些基因涉及维持多能性、早期红细胞特化、快速细胞生长以及细胞-细胞粘附和相互作用。我们还发现了每个转变中的动态网络及其中心节点。我们的研究为进一步研究红细胞分化和发育提供了基础，并对使用 ESERs 作为临床输血产品具有重要意义。
