Zimmermann W, Chen S M, Bolden A, Weissbach A
J Biol Chem. 1980 Dec 25;255(24):11847-52.
Aphidicolin, a known inhibitor of DNA polymerase alpha, is a potent inhibitor of nuclear DNA synthesis in HeLa cells but has no effect on the replication of mitochondrial DNA. Parallel experiments with mitochondria incubated in vitro also show no inhibition of DNA synthesis by aphidicolin; however, DNA synthesis in these isolated mitochondria is completely blocked by dideoxycytidine triphosphate, which inhibits DNA polymerase gamma but not the alpha polymerase. The replication of mitochondrial DNA therefore requires only one DNA polymerase of the gamma type.
阿非迪霉素是一种已知的DNA聚合酶α抑制剂，它能有效抑制HeLa细胞中的核DNA合成，但对线粒体DNA的复制没有影响。对体外培养的线粒体进行的平行实验也表明，阿非迪霉素不会抑制DNA合成；然而，这些分离出线粒体中的DNA合成会被三磷酸双脱氧胞苷完全阻断，三磷酸双脱氧胞苷抑制DNA聚合酶γ而不抑制α聚合酶。因此，线粒体DNA的复制仅需要一种γ型DNA聚合酶。
