庆大霉素生物自显影测定法与微生物纸片扩散法的比较

Gentamicin bioautography assay vs. the microbiological disk test.

作者信息

Rosner A, Aviv H

出版信息

J Antibiot (Tokyo). 1980 Jun;33(6):600-3. doi: 10.7164/antibiotics.33.600.

DOI:10.7164/antibiotics.33.600

Abstract

The use of bioautography for quantitative measurement of gentamicin concentrations was compared with the disk test. Following chromatographic separation and bioautography, gentamicin produced inhibition zones, 2 approximately 7 times larger than the inhibition zones formed by the same amounts of gentamicin in the disk test. Bioautography, therefore, is a more sensitive assay method.

摘要

将生物自显影法用于庆大霉素浓度的定量测定，并与纸片扩散法进行比较。经色谱分离和生物自显影后，庆大霉素产生的抑菌圈比纸片扩散法中相同量庆大霉素所形成的抑菌圈大约大7倍。因此，生物自显影法是一种更灵敏的检测方法。