庆大霉素复合物的差异色谱生物测定法。

Differential chromatographic bioassay for the gentamicin complex.

作者信息

Wagman G H, Oden E M, Weinstein M J

出版信息

Appl Microbiol. 1968 Apr;16(4):624-7. doi: 10.1128/am.16.4.624-627.1968.

DOI:10.1128/am.16.4.624-627.1968

PMID:5647523

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC547482/

Abstract

A differential paper chromatographic assay for the components of the gentamicin complex, designated as C(1), C(1a), and C(2), is described. The gentamicins are separated on paper strips with the lower phase of a solvent mixture consisting of chloroform, methanol, and 17% ammonium hydroxide (2: 1: 1, v/v). After chromatographic separation, the paper strips are plated against Staphylococcus aureus ATCC 6538P, and the zones of activity are quantified against known standards.

摘要

本文描述了一种用于庆大霉素复合物中C(1)、C(1a)和C(2)成分的差异纸色谱分析法。庆大霉素在由氯仿、甲醇和17%氢氧化铵（2:1:1，v/v）组成的溶剂混合物的下层相的纸条上进行分离。色谱分离后，将纸条接种于金黄色葡萄球菌ATCC 6538P，根据已知标准对活性区域进行定量。