二氧化碳依赖型链球菌的生化特性。
Biochemical properties of CO2-dependent streptococci.
作者信息
Pulliam L, Porschen R K, Hadley W K
出版信息
J Clin Microbiol. 1980 Jul;12(1):27-31. doi: 10.1128/jcm.12.1.27-31.1980.
Abstract
A total of 153 clinical isolates and 10 reference strains were employed in an investigation of CO2-dependent streptococci. Their selection was based on a lactic acid homofermentative end product. This group of organisms grew best in 5 to 10% CO2, and several species, including Streptococcus mutans, S. intermedius, Streptococcus MG, S. anginosus, and S. constellatus, required increased CO2 for primary recovery. A basal medium of thioglycolate with 0.1% Tween 80 and phenol red was prepared and used with selected carbohydrates. This media provided luxuriant growth. Serological testing failed to give any definitive correlation with species identification. A shortened differentiation scheme, combined from previous studies, was proposed.
摘要
总共153株临床分离株和10株参考菌株被用于二氧化碳依赖性链球菌的研究。它们的选择基于同型乳酸发酵终产物。这组微生物在5%至10%的二氧化碳环境中生长最佳，包括变形链球菌、中间链球菌、MG链球菌、咽峡炎链球菌和星座链球菌在内的几种菌种，初次分离时需要增加二氧化碳浓度。制备了含有0.1%吐温80和酚红的硫乙醇酸盐基础培养基，并与选定的碳水化合物一起使用。这种培养基促进了丰富的生长。血清学检测未能与菌种鉴定产生任何明确的关联。结合先前的研究，提出了一种简化的鉴别方案。
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