Shockley R K, Coffee E E, Johnston K H
J Clin Microbiol. 1980 Jul;12(1):35-8. doi: 10.1128/jcm.12.1.35-38.1980.
A broth medium that supports growth of Neisseria gonorrhoeae to high densities with minimal lysis is described. With most gonococcal strains, inocula of 2 x 10(6) colony-forming units per ml yielded greater than 109 colony-forming units per ml after 8 h of incubation. Scale-up cultures produced 5 to 12 g (wet weight) of cells per liter.
本文描述了一种肉汤培养基,该培养基能支持淋病奈瑟菌高密度生长,且细胞裂解最少。对于大多数淋球菌菌株,每毫升接种2×10⁶个菌落形成单位,培养8小时后每毫升可产生超过10⁹个菌落形成单位。放大培养每升可产生5至12克(湿重)细胞。