Yoshida H, Fukunishi R, Kato Y, Matsumoto K
J Natl Cancer Inst. 1980 Oct;65(4):823-8. doi: 10.1093/jnci/65.4.823.
Progesterone stimulated the growth of mammary carcinomas previously induced by 7,12-dimethylbenz[a]anthracene (DMBA) in neonatally androgenized female Sprague-Dawley rats. This stimulatory effect appeared to depend on the action of estrogens. At 2 days of age, 199 female rats were neonatally androgenized by single sc injections of 1.25 mg testosterone propionate. At 50 days of age, all rats were given 20-mg doses of DMBA by gastric intubation. About one-half of the androgenized rats were ovariectomized at 78 days of age. Rats in test groups were given daily im injections of 10 micrograms 17 beta-estradiol or 4 mg progesterone for 228 days starting at 78 days after birth. The experiments were terminated when the rats were 300 days of age. In all androgenized rats, corpora lutea were absent. In the endrogenized rats, progesterone injection induced a significant and marked increase in the incidence of mammary carcinomas from that in the androgenized controls (3.4%) in the presence of ovaries (90%) but not in the absence of ovaries (8.7%). Estrogen injecton showed some but no significant increases in the incidence of mammary carcinoma in both ovariectomized and nonovariectomized androgenized rats (17.4 and 40.0%, respectively).
孕酮可刺激新生期经雄激素化的雌性斯普拉格 - 道利大鼠体内先前由7,12 - 二甲基苯并[a]蒽（DMBA）诱导产生的乳腺癌生长。这种刺激作用似乎依赖于雌激素的作用。在2日龄时，199只雌性大鼠通过单次皮下注射1.25毫克丙酸睾酮进行新生期雄激素化。在50日龄时，所有大鼠通过胃内插管给予20毫克剂量的DMBA。约一半的雄激素化大鼠在78日龄时进行卵巢切除。试验组的大鼠从出生后78天开始，每天肌肉注射10微克17β - 雌二醇或4毫克孕酮，持续228天。当大鼠300日龄时实验结束。在所有雄激素化大鼠中，均未见黄体。在雄激素化大鼠中，注射孕酮会使有卵巢组的乳腺癌发生率相比雄激素化对照组（3.4%）显著且明显增加（90%），但在无卵巢组（8.7%）中则无此现象。在切除卵巢和未切除卵巢的雄激素化大鼠中，注射雌激素均使乳腺癌发生率有一定增加，但无显著差异（分别为17.4%和40.0%）。
