Mouse-protection experience with monovalent Pseudomonas aeruginosa vaccine.

The protective effect of the soluble immunogenic complex from Pseudomonas aeruginosa cell surface was studied on white mice. Vaccines were prepared from a single strain by phenol-water extraction or via preparing the Zn-complex. The immunogenic complex which contained the Pseudomonas "common protective antigen" as well as endotoxin and exotoxins in the form of toxoid, provided reliable protection if it was administered with aluminium hydroxide as adjuvant in three consecutive doses of 10, 100 and 500 microgram per 0.2 ml per mouse at 5-7 day intervals. Under these conditions, the monovaccine produced in mice a 90-100% protective effect against a lethal challenge dose (2 LD100) of toxic P. aeruginosa strains given a week after the last vaccine dose.