Reybrouck G
Zentralbl Bakteriol B Hyg Krankenhaushyg Betriebshyg Prav Med. 1980;170(5-6):449-56.
The germicidal effect of two disinfectant standards has been determined by three quantitative suspension tests: the Dutch Standard-Suspension Test, the French AFNOR test, and the in vitro test. Particularly in case of Pseudomonas aeruginosa the germicidal-effect values found according to the three methods differ in a significant way (Tables 1 and 2). The only factors that could explain these differences, since they are not the same in the three techniques, are the preparation of the bacterial suspension and the diluent of the disinfectant solution. Through this the composition of the reaction mixture is different: in the in vitro test only 0.0085% NaC1 and 0.001% Tryptone are contained, in the AFNOR test the same constituants are ten times more concentrated and in the Standard-Suspension Test 0.882% NaC1 and 0.0306% albumin are present. If in the same trial these substances have been incorporated in the final concentration above-mentioned, then germicidal-effect values equal to those of the original tests have been obtained (Table 3). The disinfectant diluent and the bacterial suspension fluid have to be the main factors responsible for the differences in activity found by the three testing methods. In our opinion the in vitro test gives the clearest picture of the anti-bacterial properties of a disinfectant since in this test the less addition of extraneous matter is encountered.
荷兰标准悬液试验、法国AFNOR试验和体外试验。特别是对于铜绿假单胞菌，根据这三种方法得出的杀菌效果值存在显著差异（表1和表2）。由于这三种技术中这些因素不尽相同，所以唯一能解释这些差异的因素就是细菌悬液的制备和消毒剂溶液的稀释剂。由此反应混合物的组成不同：体外试验中仅含有0.0085%的NaCl和0.001%的胰蛋白胨，在AFNOR试验中相同成分的浓度高十倍，而在标准悬液试验中有0.882%的NaCl和0.0306%的白蛋白。如果在同一试验中将这些物质以上述最终浓度加入，那么就会得到与原始试验相同的杀菌效果值（表3）。消毒剂稀释剂和细菌悬液必定是造成三种测试方法所发现的活性差异的主要因素。我们认为体外试验能最清晰地展现消毒剂的抗菌特性，因为在该试验中遇到的外来物质添加较少。
