Reybrouck G
Zentralbl Bakteriol Orig B. 1975 Jul;160(4-5):342-67.
The purpose of this study is to compare six methods of disinfectant testing, currently applied in Europe. The testing techniques are analysed into the separate elements, which can influence the activity of the disinfectant. The differences observed in these components allow to explain why in each method other parameters are evaluated and consequently, why the interpretation of the results varies from method to method. The six tests examined are (1) the suspension test of the DGHM, (2) the suspension test of the Committee on Phytopharmacy, (3) the A.O.A.C. use-dilution method, (4) the KELSEY SYKES test, (5) the surface-disinfection test of the DGHM and (6) a modified version of this latter. First of all the final concentrations of the disinfectant in the medication mixture are calculated. On the KELSEY SYKES test differs from the others, in using a much lower final dilution, up to 50% in the 3 rd stage. It is also obvious that the longer the exposure time is, the greater the killing effect on the bacteria in the medication mixture will be. To determine the germicidal activity a method needs to pass the test, the germicidal effect has been assessed starting from the inoculum applied in the medication mixture and, in case of tests that consider the absence of growth in the subcultures as end-point, the minimum extinction required. The lowest number of bacteria is applied in the inoculum of the A.O.A.C. use-dilution method (ca. 10(6)) and the highest in the modified practical surface disinfection test (ca. 10(10)). So the required germicidal effect varies from 5 (in case of the Committee on Phytopharmacy's test and the modified practical test) to more than 8 (in case of the A.O.A.C. use-dilution method). Further on, the protein content of the bacterial suspension which is added to the medication mixture, is experimentically verified. Considerable differences are observed here. In some tests the presence of organic matter is negligible (the A.O.A.C. use-dilution method, the KELSEY SYKES test under clean conditions), whereas in others (the KELSEY SYKES test under dirty conditions and the practical surface disinfection tests) the protein added equals that of serum 10-30%. Finally it is mentioned that the dilutions in the KELSEY SYKES test are made with artificial hard water. From a comparison of the factors described above it clearly appears that in the distinct tests different parameters are used and that the results of one test cannot be applied to another.
本研究的目的是比较目前在欧洲应用的六种消毒剂测试方法。将测试技术分解为可影响消毒剂活性的各个要素。在这些要素中观察到的差异有助于解释为何在每种方法中评估的是其他参数，以及为何不同方法对结果的解释各不相同。所考察的六种测试分别是：（1）德国卫生与微生物学会（DGHM）的悬液测试；（2）植物药剂委员会的悬液测试；（3）美国官方分析化学师协会（A.O.A.C.）的使用稀释法；（4）凯尔西 - 赛克斯（KELSEY SYKES）测试；（5）DGHM的表面消毒测试；（6）后者的改良版本。首先计算药物混合物中消毒剂的最终浓度。凯尔西 - 赛克斯测试与其他测试不同，它使用的最终稀释度低得多，在第三阶段可达50%。同样明显的是，暴露时间越长，对药物混合物中细菌的杀灭效果就越大。为了确定杀菌活性，一种方法需要通过测试，杀菌效果是从药物混合物中接种物开始评估的，对于将亚培养物中无生长作为终点的测试，则是评估所需的最低杀灭率。美国官方分析化学师协会使用稀释法接种物中的细菌数量最少（约10⁶），改良的实际表面消毒测试中最多（约10¹⁰）。因此，所需的杀菌效果从5（植物药剂委员会测试和改良实际测试的情况）到超过8（美国官方分析化学师协会使用稀释法的情况）不等。此外，对添加到药物混合物中的细菌悬液的蛋白质含量进行了实验验证。在此观察到显著差异。在一些测试中，有机物的存在可忽略不计（美国官方分析化学师协会使用稀释法、清洁条件下的凯尔西 - 赛克斯测试），而在其他测试中（脏污条件下的凯尔西 - 赛克斯测试和实际表面消毒测试），添加的蛋白质相当于血清的10 - 30%。最后提到，凯尔西 - 赛克斯测试中的稀释是用人工硬水进行的。从上述因素的比较中可以清楚地看出，在不同的测试中使用了不同的参数，一种测试的结果不能应用于另一种测试。
