van Klingeren B
Zentralbl Bakteriol Mikrobiol Hyg B. 1982 Apr;176(1):63-71.
Notwithstanding considerable international agreement upon the principle of the quantitative suspension test differences still exist between the main variants of this test, in particular on the diluent to be used in the test mixture. For this reason the influence of the main variables - i.e. bovine albumin (0.03%), NaCl (physiol. saline) and divalent ions (water of standard hardness) - on the activity of the different classes of disinfectants have been investigated. Using so-called borderline concentrations only a small protein effect of 0.03% bovine albumin was found with a phenolic product, quaternary ammonium compounds and aldehydes. A substantial protein effect was observed only with chlorine compounds. The chlorine consumption in the present Dutch Standard Suspension Test is approx. 20 ppm. In tests without a protein load as well as in the presence of 0.03% bovine albumin M.E.-values using saline as the diluent were usually higher than in tests using water of standard hardness (WSH) or distilled water (DW). M.E.-values of quaternary ammonium compounds in WSH were significantly lower than those in DW, presumably due to the antagonizing effect of divalent cations (Mg++ and Ca++). The different views regarding the aim of quantitative suspension tests are discussed. In The Netherlands the opinion prevails that these tests should have at least some relationship with practical conditions. The use of water of standard hardness as a diluent and the incorporation of a slight protein challenge are in agreement with this standpoint.
尽管在定量悬液试验原理上存在相当多的国际共识，但该试验的主要变体之间仍存在差异，特别是在试验混合物中使用的稀释剂方面。因此，研究了主要变量，即牛血清白蛋白（0.03%）、氯化钠（生理盐水）和二价离子（标准硬度水）对不同类别消毒剂活性的影响。仅使用所谓的临界浓度时，发现0.03%牛血清白蛋白对酚类产品、季铵化合物和醛类仅有轻微的蛋白质效应。仅在含氯化合物中观察到显著的蛋白质效应。在当前荷兰标准悬液试验中，氯的消耗量约为20 ppm。在无蛋白质负载的试验以及存在0.03%牛血清白蛋白的试验中，以生理盐水作为稀释剂时的M.E.值通常高于使用标准硬度水（WSH）或蒸馏水（DW）的试验。WSH中季铵化合物的M.E.值显著低于DW中的值，这可能是由于二价阳离子（Mg++和Ca++）的拮抗作用。讨论了关于定量悬液试验目的的不同观点。在荷兰，普遍认为这些试验应至少与实际情况有一定关联。使用标准硬度水作为稀释剂以及加入轻微的蛋白质挑战符合这一观点。
