Bosseray N
Br J Exp Pathol. 1980 Aug;61(4):361-8.
Pregnant mice were challenged at Day 3, 7, 11 or 15 of pregnancy with Brucella abortus Strain 544 and killed at Day 18 of pregnancy for the enumeration of brucella in the spleens and individual placentas. Whatever the route of challenge--i.p., i.v. or s.c. into the foot-pad (F-s.c.) no abortions or foetal deaths were observed. Placental colonization involved either all, none or only some of the placentas in the same uterus (partial placental colonization: 25% of the mice). In the latter case, the probability of colonization was the same for all sites of implantation. Placentas were independent units as regards colonization and bacterial proliferation. Placental colonization was expressed either by (1) the class of placental infection within the uterus, which might be total, partial or nil; (2) the ratio of infected to total placentas analysed per group; or (3) the mean degree of infection per group. Whatever means of expression was chosen, placental colonization increased with the dose of challenge in parallel with splenic infection in the mouse. The challenge doses required at Day 7 to infect 50% of the placentas differed according to the route (i.p. = 54; i.v. = 5.6 x 10(2) and F-s.c. = 3.6 x 10(4) brucella). Placentas were more frequently and more intensively colonized when the challenge was performed at Days 7 and 11 than at Days 3 and 15 at pregnancy. Mice immunized with H.38 B. melitensis killed vaccine 36 days before pregnancy were found to be protected against an i.p. challenge with 2 x 10(5) brucella at Day 7 of pregnancy.
在妊娠第3、7、11或15天，用流产布鲁氏菌544菌株对怀孕小鼠进行攻击，并在妊娠第18天处死小鼠，以计数脾脏和单个胎盘中的布鲁氏菌。无论攻击途径如何——腹腔注射、静脉注射或足垫皮下注射（F-s.c.），均未观察到流产或胎儿死亡。胎盘定植涉及同一子宫内的所有、无或仅部分胎盘（部分胎盘定植：25%的小鼠）。在后一种情况下，所有着床部位的定植概率相同。胎盘在定植和细菌增殖方面是独立的单位。胎盘定植通过以下方式表示：（1）子宫内胎盘感染的类别，可能是全部、部分或无；（2）每组分析的感染胎盘与总胎盘的比例；或（3）每组的平均感染程度。无论选择何种表达方式，胎盘定植均随攻击剂量的增加而增加，与小鼠脾脏感染情况平行。在第7天感染50%胎盘所需的攻击剂量因途径而异（腹腔注射=54；静脉注射=5.6×10²，F-s.c.=3.6×10⁴布鲁氏菌）。与妊娠第3天和15天相比，在妊娠第7天和11天进行攻击时，胎盘更频繁、更密集地被定植。发现妊娠前36天用H.38 羊种布鲁氏菌灭活疫苗免疫的小鼠在妊娠第7天能抵抗2×10⁵布鲁氏菌的腹腔攻击。
