Lynn R J, Guidici P A, Hollemweguer E J, Smith P F
Infect Immun. 1980 Sep;29(3):926-33. doi: 10.1128/iai.29.3.926-933.1980.
Lipoglycans extracted from Acholeplasma species with hot aqueous phenol were immunogenic for rabbits when introduced by an appropriate method. All lipoglycans examined elicited antibody associated with a heavy, 2-mercaptoethanol-sensitive immunoglobulin fraction when inoculated intravenously adsorbed to autologous rabbit erythrocytes. This antibody was specific for the Acholeplasma species from which the lipoglycan was extracted. Extensive immunization of these animals with acholeplasmal lipoglycans produced significant increases in sheep erythrocyte hemolysin. Some, but not all, Acholeplasma species yielded lipoglycans that were immunogenic when emulsified with Freund complete adjuvant and introduced via the footpad into rabbits. Such animals produced antibodies corresponding to the M and G immunoglobulin classes that reacted with both homologous and heterologous acholeplasmal lipoglycans by precipitation in immunodiffusion as well as passive hemagglutination. None of the animals inoculated demonstrated a significant anamnestic response after booster injections either intravenously or via the footpads.
用热酚水溶液从无胆甾原体属中提取的脂聚糖，通过适当方法引入时，对兔子具有免疫原性。所有检测的脂聚糖静脉注射吸附到自体兔红细胞上时，都会引发与重链、对2 - 巯基乙醇敏感的免疫球蛋白组分相关的抗体。这种抗体对提取脂聚糖的无胆甾原体属具有特异性。用无胆甾原体脂聚糖对这些动物进行广泛免疫，会使绵羊红细胞溶血素显著增加。一些（但不是全部）无胆甾原体属产生的脂聚糖，与弗氏完全佐剂乳化并经足垫引入兔子时具有免疫原性。这类动物产生对应于M和G免疫球蛋白类别的抗体，这些抗体通过免疫扩散沉淀以及被动血凝反应，与同源和异源无胆甾原体脂聚糖发生反应。无论是静脉注射还是经足垫进行加强注射后，接种的动物均未表现出明显的回忆反应。
