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颗粒无胆甾原体和黄色无胆甾原体脂聚糖上主要抗原决定簇的鉴定。

Identification of the major antigenic determinants on lipoglycans from Acholeplasma granularum and Acholeplasma axanthum.

作者信息

Al-Samarrai T H, Smith P F, Lynn R J

出版信息

Infect Immun. 1983 May;40(2):629-32. doi: 10.1128/iai.40.2.629-632.1983.

Abstract

The major antigenic determinants of the lipoglycans from Acholeplasma granularum and Acholeplasma axanthum were found to be the oligosaccharide sequences Glcp(beta 1 leads to 2)-Glcp(alpha 1 leads to 4)-Glcp and Glcp(beta 1 leads to 2)-Glcp(beta 1 leads to 2)-Glcp, respectively. The disaccharides sophorose and maltose inhibited hemagglutination by specific antiserum of sheep erythrocytes coated with lipoglycan from A. granularum. Only sophorose possessed this capacity with the lipoglycan from A. axanthum. Periodate oxidation destroyed the ability of both lipoglycans to interact with specific antibody, a result compatible with structural data on the lipoglycans.

摘要

研究发现,颗粒无胆甾原体和黄色无胆甾原体脂多糖的主要抗原决定簇分别是寡糖序列Glcp(β1→2)-Glcp(α1→4)-Glcp和Glcp(β1→2)-Glcp(β1→2)-Glcp。二糖槐糖和麦芽糖可抑制颗粒无胆甾原体脂多糖包被的绵羊红细胞特异性抗血清的血凝作用。只有槐糖对黄色无胆甾原体脂多糖具有这种作用。高碘酸盐氧化破坏了两种脂多糖与特异性抗体相互作用的能力,这一结果与脂多糖的结构数据相符。

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