脊椎动物中玻尔效应动力学的比较研究。
A comparative study of the kinetics of the Bohr effect in vertebrates.
作者信息
Maren T H, Swenson E R
出版信息
J Physiol. 1980 Jun;303:535-47. doi: 10.1113/jphysiol.1980.sp013302.
Abstract
- The kinetics of the Bohr effect and the role of carbonic anhydrase were studied in a series of representative vertebrates using a continuous flow rapid reaction apparatus. 2. The rates of the Bohr effect in vertebrates are very similar, and differences among classes are manifestations of the ambient temperature. 3. Complete carbonic anhydrase inhibition causes a fifteen to fortyfold reduction in the rate of the Bohr effect, sufficient to abolish its occurrence within capillary transit. 4. There is a twenty-three-fold (duck) to 360-fold (man) excess of carbonic anhydrase activity in vertebrate red cells for the normal generation of the Bohr effect. 5. When carbonic anhydrase is inhibited and CO2 hydration becomes rate limiting, the stoichiometry of the Bohr effect (delta log pO2/delta pH) is revealed in the ratio of the rates of proton formation in red cells to O2 release from haemoglobin.
摘要
- 使用连续流动快速反应装置，在一系列有代表性的脊椎动物中研究了波尔效应的动力学及碳酸酐酶的作用。2. 脊椎动物中波尔效应的速率非常相似，不同类别之间的差异是环境温度的表现。3. 完全抑制碳酸酐酶会使波尔效应的速率降低15至40倍，足以在毛细血管通过期间消除其发生。4. 脊椎动物红细胞中碳酸酐酶活性超过正常产生波尔效应所需量23倍（鸭）至360倍（人）。5. 当碳酸酐酶被抑制且二氧化碳水合成为限速步骤时，波尔效应的化学计量（δlog pO2/δpH）在红细胞中质子形成速率与血红蛋白释放氧气速率的比值中得以体现。
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