Parra F, Moreno F, Herrero P, Gascon S
Biochim Biophys Acta. 1980 Nov 14;610(1):141-6. doi: 10.1016/0005-2787(80)90063-5.
When Saccharomyces carlsbergensis G-517 was grown in 10 mM galactose as the carbon source, the addition of 2-deoxy-D-glucose restricted the uptake of galactose, [3H]uridine and [3H]leucine, and restricted invertase synthesis (beta-D-fructofuranoside fructohydrolase; EC 3.2.1.26) for a period of 60-90 min. During this time, the radioactive antimetabolite was taken up by the cells; afterwards, invertase synthesis was enhanced, and the utilizaton rate of galactose, [3H]uridine and [3H]leucine increased until it reached that of the control culture. When glucose was used as a carbon source, sugar utilization and uptake of radioactive precursors were unaffected by addition of the deoxysugar.
当将卡尔斯伯酵母G-517在10 mM半乳糖作为碳源的条件下培养时，添加2-脱氧-D-葡萄糖会在60 - 90分钟内限制半乳糖、[³H]尿苷和[³H]亮氨酸的摄取，并限制转化酶（β-D-呋喃果糖苷果糖水解酶；EC 3.2.1.26）的合成。在此期间，放射性抗代谢物被细胞摄取；之后，转化酶合成增强，半乳糖、[³H]尿苷和[³H]亮氨酸的利用率增加，直至达到对照培养物的水平。当使用葡萄糖作为碳源时，脱氧糖的添加不会影响糖的利用和放射性前体的摄取。
