Toda K
Biotechnol Bioeng. 1976 Aug;18(8):1103-15. doi: 10.1002/bit.260180807.
The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis at a particular intermediate growth rate. The value of E/X increased from 1.1 (U/mg biomass) in batch cultures to 13 (U/mg biomass) in chemostat cultures. A mutant strain A3 showed the highest value for E/X = 25 (U/mg biomass) at high dilution rates where glucose repression was observed with the wild strain.
研究了卡尔斯伯酵母LAM 1068转化酶的生物合成与生长非稳态和稳态下葡萄糖效应的关系。在恒化器培养中，稀释率与转化酶比活性（E/X）之间的实验相关性表明，在特定的中间生长速率下酶合成达到最佳。E/X的值从分批培养中的1.1（U/mg生物量）增加到恒化器培养中的13（U/mg生物量）。突变菌株A3在高稀释率下显示出最高的E/X值，为25（U/mg生物量），而野生菌株在该稀释率下存在葡萄糖阻遏现象。
