Lernau O Z, Nissan S, Neufeld B, Mayer M
Eur J Clin Invest. 1980 Oct;10(5):357-61. doi: 10.1111/j.1365-2362.1980.tb00045.x.
Myofibrillar alkaline protease activity was shown to be present in human skeletal muscle. Endogenous myofibrillar proteins and 14C-labelled, exogenous haemoglobin were both active as substrates, and the enzymic activity appeared to be similar to the myofibrillar protease previously described in rodent muscles. The activity of this enzyme was determined in patients undergoing surgery for a variety of diseases. Significant elevations in proteolytic activity were found in the abdominal wall muscle of patients in wasting conditions as compared with non-catabolic diseases. In cachectic patients on total parenteral nutrition the protease activity was similar to reference values. The results imply that increased activity of the myofibrillar alkaline protease plays a role in the development of cachexia in human wasting diseases by prompting degradation of muscle proteins.
肌原纤维碱性蛋白酶活性在人体骨骼肌中被证实存在。内源性肌原纤维蛋白和14C标记的外源性血红蛋白均作为底物具有活性，并且这种酶活性似乎与先前在啮齿动物肌肉中描述的肌原纤维蛋白酶相似。在因各种疾病接受手术的患者中测定了这种酶的活性。与非分解代谢疾病患者相比，处于消瘦状态的患者腹壁肌肉中的蛋白水解活性显著升高。在接受全胃肠外营养的恶病质患者中，蛋白酶活性与参考值相似。这些结果表明，肌原纤维碱性蛋白酶活性的增加通过促使肌肉蛋白降解，在人类消瘦性疾病恶病质的发展中起作用。
