Collins M L, Salton M R
Infect Immun. 1980 Oct;30(1):281-8. doi: 10.1128/iai.30.1.281-288.1980.
Cell envelopes were obtained from lysates of Neisseria gonorrhoeae, colony type T1, prepared with lysozyme, ethylenediaminetetraacetate, and Brij 58. This preparation was separated into cytoplasmic (inner) and outer membrane fractions by equilibrium sucrose density gradient centrifugation. The former fraction was 10-fold enriched in L-lactate dehydrogenase activity with respect to the latter. On the basis of buoyant density in sucrose, polypeptide patterns in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and enzymatic activity, these preparations appear similar to cytoplasmic and outer membrane preparations from other gram-negative bacteria. The membrane preparations were analyzed by high-resolution crossed immunoelectrophoretic procedures. This technique permitted the identification of antigens originating from the structural components of the gonococcal cell. Among those found to be cytoplasmic membrane components was the fast-moving antigen which occurs widely in gram-negative bacteria.
从淋病奈瑟菌T1菌落型菌株的裂解物中获取细胞包膜，该裂解物用溶菌酶、乙二胺四乙酸和Brij 58制备。通过平衡蔗糖密度梯度离心将该制剂分离为细胞质（内膜）和外膜部分。相对于后者，前一部分的L-乳酸脱氢酶活性富集了10倍。基于蔗糖中的浮力密度、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的多肽模式和酶活性，这些制剂似乎与其他革兰氏阴性菌的细胞质和外膜制剂相似。通过高分辨率交叉免疫电泳程序分析膜制剂。该技术能够鉴定源自淋球菌细胞结构成分的抗原。在那些被发现是细胞质膜成分的抗原中，有一种快速移动的抗原，它广泛存在于革兰氏阴性菌中。
