Dempsey S I, Crockard A D, Bridges J M
Acta Haematol. 1980;64(3):141-7. doi: 10.1159/000207227.
The lysosomal enzymes beta-glucuronidase (EC 3.2.1.31) and N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30) were estimated by biochemical and cytochemical means in the circulating lymphocytes of 20 control subjects, 19 cases of B cell and one case of T cell chronic lymphocytic leukaemia. Significantly lower levels of activity were observed both biochemically and cytochemically for each enzyme in B cell chronic lymphocytic leukaemia lymphocytes, whereas the values obtained for the T cell chronic lymphocytic leukaemic patient fell within the normal range. The absence of staining reaction for both enzymes in the majority of B cell chronic lymphocytic leukemia lymphocytes contrasted with the uniform pattern of polar positivity obtained in T cell chronic lymphocytic leukaemia lymphocytes.
通过生化和细胞化学方法，对20名对照受试者、19例B细胞慢性淋巴细胞白血病患者及1例T细胞慢性淋巴细胞白血病患者循环淋巴细胞中的溶酶体酶β-葡萄糖醛酸酶（EC 3.2.1.31）和N-乙酰-β-D-氨基葡萄糖苷酶（EC 3.2.1.30）进行了测定。在B细胞慢性淋巴细胞白血病淋巴细胞中，两种酶的生化活性和细胞化学活性均显著降低，而T细胞慢性淋巴细胞白血病患者所测值则在正常范围内。大多数B细胞慢性淋巴细胞白血病淋巴细胞中两种酶均无染色反应，这与T细胞慢性淋巴细胞白血病淋巴细胞中极性阳性的均匀模式形成对比。
