Schmidt S, Wilke B, Ziegler B, Jahr H, Zühlke H
Endokrinologie. 1980 Oct;76(2):163-70.
An insulin response to increased glucose concentrations could not be found in vivo and in vitro after long-term treatment of C57BL/KsJ and C57BL/6J mice with Glibenclamide. This missing stimulation of insulin secretion was not the result of an exhaustion of the islets or a disturbed (pro)insulin biosynthesis as demonstrated by measurements of insulin content of the islets and by in vitro (pro)insulin biosynthesis experiments. In the presence of glucose (15 mmol/l) theophylline increased the insulin secretion of isolated islets of Glibenclamide-treated mice to values similar to control islets. The insulin response to an i.p. glucose loading was found to be normal in comparison with control mice 1-2 weeks after the Glibenclamide treatment had been finished.
在用格列本脲长期治疗C57BL/KsJ和C57BL/6J小鼠后，在体内和体外均未发现对葡萄糖浓度升高的胰岛素反应。胰岛胰岛素含量的测量以及体外（前）胰岛素生物合成实验表明，这种胰岛素分泌刺激的缺失并非胰岛耗竭或（前）胰岛素生物合成紊乱的结果。在存在葡萄糖（15 mmol/l）的情况下，茶碱可将格列本脲治疗小鼠的分离胰岛的胰岛素分泌增加至与对照胰岛相似的值。在格列本脲治疗结束1至2周后，与对照小鼠相比，腹腔注射葡萄糖负荷的胰岛素反应正常。
