Thorne C B
Appl Environ Microbiol. 1978 Jun;35(6):1109-15. doi: 10.1128/aem.35.6.1109-1115.1978.
Bacteriophage CP-51, originally reported as a generalized transducing phage for Bacillus cereus and B. anthracis, has been shown to carry out generalized transduction in several strains of B. thuringiensis. A newly isolated phage, CP-54, which has a broader host range than CP-51, also mediates generalized transduction in B. thuringiensis. CP-51 and CP-54 are similar in size and morphology and are related serologically, but they are not identical. CP-54 is more cold labile than CP-51, and, as with CP-51, its stability both at 0 and 15 degrees C is enhanced by the presence of 0.02 M Mg2+. Some examples of cotransduction of linked markers in B. thuringiensis are presented, demonstrating the feasibility of chromosomal mapping in this organism. The rare occurrence of cross-transduction among strains of B. thuringiensis is probably a reflection of nonhomology rather than restriction, since phage itself did not appear to be restricted when grown on a particular host and assayed with other hosts as indicator.
噬菌体CP-51最初被报道为蜡状芽孢杆菌和炭疽芽孢杆菌的一种广义转导噬菌体，现已证明它能在几种苏云金芽孢杆菌菌株中进行广义转导。一种新分离的噬菌体CP-54，其宿主范围比CP-51更广，也能在苏云金芽孢杆菌中介导广义转导。CP-51和CP-54在大小和形态上相似，血清学上相关，但并不相同。CP-54比CP-51更不耐冷，并且与CP-51一样，在0.02 M Mg2+存在时，其在0℃和15℃的稳定性都会增强。本文给出了苏云金芽孢杆菌中连锁标记共转导的一些例子，证明了在该生物体中进行染色体作图的可行性。苏云金芽孢杆菌菌株间罕见的交叉转导现象可能反映的是非同源性而非限制作用，因为当噬菌体在特定宿主上生长并用其他宿主作为指示物进行检测时，噬菌体本身似乎并未受到限制。
