Baresi L, Mah R A, Ward D M, Kaplan I R
Appl Environ Microbiol. 1978 Jul;36(1):186-97. doi: 10.1128/aem.36.1.186-197.1978.
An acetate enrichment culture was initiated by inoculating anaerobic sludge from a mesophilic methane digestor into a mineral salts medium with calcium acetate as the sole carbon and energy source. This enrichment was maintained indefinitely by weekly transfer into medium of the same composition. A study of this enrichment disclosed an unexpected age-dependent inhibition of methanogenesis by H2 and formate which apparently differed from the inhibition by chloroform and benzyl viologen. This age-dependent inhibition indicated that microbial interactions of the mixed enrichment population may play a regulatory role in methane formation. Futhermore, stimulation of methanogenesis in the acetate enrichment by addition of yeast extract showed a nutrient limitation which indicated that syntrophic interactions leading to formation of growth factors may also occur. A model is presented to illustrate the possible interrelationships between methanogenic and nonmethanogenic bacteria in their growth and formation of methane and carbon dioxide from acetate.
通过将中温甲烷消化器中的厌氧污泥接种到以醋酸钙作为唯一碳源和能源的矿物盐培养基中，启动了一个醋酸盐富集培养物。通过每周转移到相同成分的培养基中，这种富集得以无限期维持。对这种富集的研究揭示了氢气和甲酸盐对产甲烷作用存在意想不到的年龄依赖性抑制，这显然不同于氯仿和苄基紫精的抑制作用。这种年龄依赖性抑制表明，混合富集菌群的微生物相互作用可能在甲烷形成中起调节作用。此外，添加酵母提取物对醋酸盐富集培养物产甲烷作用的刺激表明存在营养限制，这表明也可能发生导致生长因子形成的互营相互作用。提出了一个模型来说明产甲烷菌和非产甲烷菌在利用醋酸盐生长以及形成甲烷和二氧化碳过程中可能的相互关系。
