Distribution of glycosaminoglycans across the normal and the scoliotic disc.

This paper reports on our investigations of the glycosaminoglycans (GAGs) (hyaluronic acid, chondroitin-6-sulfate, and keratan sulfate) of 13 adjacent zones of normal and scoliotic discs obtained at necropsy. The isolation and separation of these GAGs on the microgram scale was achieved using the different solubilities of the calcium and cetyl pyridinium chloride complexes. In normal discs the distribution of these GAGs is symmetric about the nucleus pulposus where their concentration is highest, and lowest in the outer annulus regions. A shift in this distribution profile was observed for the GAGs of the scoliotic discs, particularly at the apex of the curve. On the concave side of this disc the chondroitin-6-sulfate was elevated relative to discs at a lower level. In those annulus zones on the convex side of the curve, the keratan sulfate and hyaluronic acid levels were elevated and chondroitin sulfate depressed. These results suggest the presence in these respective regions of at least two different species of proteoglycans accompanied by elevated hyaluronate levels.