Ghosh P, Bushell G R, Taylor T K, Pearce R H, Grimmer B J
Spine (Phila Pa 1976). 1980 Jul-Aug;5(4):310-7. doi: 10.1097/00007632-198007000-00004.
This paper reports on our investigations of the glycosaminoglycans (GAGs) (hyaluronic acid, chondroitin-6-sulfate, and keratan sulfate) of 13 adjacent zones of normal and scoliotic discs obtained at necropsy. The isolation and separation of these GAGs on the microgram scale was achieved using the different solubilities of the calcium and cetyl pyridinium chloride complexes. In normal discs the distribution of these GAGs is symmetric about the nucleus pulposus where their concentration is highest, and lowest in the outer annulus regions. A shift in this distribution profile was observed for the GAGs of the scoliotic discs, particularly at the apex of the curve. On the concave side of this disc the chondroitin-6-sulfate was elevated relative to discs at a lower level. In those annulus zones on the convex side of the curve, the keratan sulfate and hyaluronic acid levels were elevated and chondroitin sulfate depressed. These results suggest the presence in these respective regions of at least two different species of proteoglycans accompanied by elevated hyaluronate levels.
本文报道了我们对尸检时获取的正常椎间盘和脊柱侧弯椎间盘13个相邻区域的糖胺聚糖（GAGs）（透明质酸、硫酸软骨素-6和硫酸角质素）的研究。利用钙和十六烷基吡啶氯化物复合物的不同溶解度，实现了微克级这些GAGs的分离。在正常椎间盘中，这些GAGs围绕髓核呈对称分布，髓核处浓度最高，在外层纤维环区域最低。观察到脊柱侧弯椎间盘的GAGs分布曲线发生了变化，特别是在曲线顶点处。在该椎间盘的凹侧，硫酸软骨素-6相对于较低水平的椎间盘有所升高。在曲线凸侧的那些纤维环区域，硫酸角质素和透明质酸水平升高，硫酸软骨素降低。这些结果表明，在这些相应区域存在至少两种不同种类的蛋白聚糖，同时伴有透明质酸盐水平升高。
