Gill C W, Bush W S, Burleigh W M, FIscher C L
Am J Clin Pathol. 1981 Jan;75(1):50-5. doi: 10.1093/ajcp/75.1.50.
By using 20 repetitive assays at three levels of C-reactive protein control serum, within-run and day-to-day precision of a rate immunonephelometric system was established. The precision was excellent in each case. Linearity of the system between 1.8 and 20 mg/dl was established at a coefficient of correlation of 0.999 by using serial dilutions of C-reactive protein control serum. When the immunonephelometric assay was compared with electroimmunodiffusion and radial immunodiffusion assays, coefficients of correlation of 0.986 and 0.993, respectively, were obtained. The lower limit of sensitivity of the immunonephelometric assay was 1.8 mg/dl. The relationship of clinical significance to the performance of the C-reactive protein test system using rate immunonephelometry is discussed.
通过在三个C反应蛋白对照血清水平上进行20次重复测定，建立了速率免疫比浊系统的批内和日间精密度。在每种情况下，精密度都非常出色。通过对C反应蛋白对照血清进行系列稀释，在1.8至20 mg/dl之间建立了该系统的线性关系，相关系数为0.999。当将免疫比浊法与电免疫扩散法和放射免疫扩散法进行比较时，分别获得了0.986和0.993的相关系数。免疫比浊法的灵敏度下限为1.8 mg/dl。讨论了速率免疫比浊法C反应蛋白检测系统的临床意义与性能之间的关系。
