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对属于嗜热栖热菌属的极端嗜热细菌的比较分析。

A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus.

作者信息

Degryse E, Glansdorff N, Piérard A

出版信息

Arch Microbiol. 1978 May 30;117(2):189-96. doi: 10.1007/BF00402307.

DOI:10.1007/BF00402307
PMID:678024
Abstract

Several extreme thermophilic Gram negative bacteria found in a thermally polluted river in Belgium have been compared with Thermus strains isolated from widely distant geographical areas. This analysis has become possible after the design of a new culture medium (162). All strains examined (including the isolate successively denominated Flavobacterium thermophilum and Thermus thermophilus) were found to be morphologically identical with strain YT-1 of Thermus aquaticus. The cells are immotile, rod-like, strictly aerobic, catalase and oxidase positive. They produce amylase, hydrolyze gelatin and are confirmed to be highly sensitive towards penicillin. The nutritional pattern of all strains has been analysed extensively, by testing a broad spectrum of possible substrates. The strains display a uniform response to the microbiological tests applied and most probably belong to the same species: Thermus aquaticus.

摘要

在比利时一条受热污染的河流中发现的几种极端嗜热革兰氏阴性菌,已与从相距甚远的地理区域分离出的嗜热栖热菌菌株进行了比较。在设计出一种新的培养基(162)之后,这种分析才得以进行。所有检测的菌株(包括先后命名为嗜热黄杆菌和嗜热栖热菌的分离株)在形态上都与嗜热水栖热菌的YT-1菌株相同。细胞无运动性,呈杆状,严格需氧,过氧化氢酶和氧化酶呈阳性。它们能产生淀粉酶,水解明胶,并且已证实对青霉素高度敏感。通过测试广泛的可能底物,对所有菌株的营养模式进行了广泛分析。这些菌株对所应用的微生物学测试表现出一致的反应,很可能属于同一物种:嗜热水栖热菌。

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